Our results provide insights into how the enzyme recognizes and depolymerizes the K1 CPS, and demonstrate the potential use of the protein not only as a therapeutic agent against K. pneumoniae infection, even against a high challenge dose. We also demonstrate that the enzyme is capable of protecting mice from K1 K. We demonstrate the tight interaction between the pyruvate moiety of K1 CPS and the enzyme in this second carbohydrate-binding site to be crucial to CPS depolymerization of the enzyme as well as phage absorption and infectivity. We also determined the 1.46-Å-resolution, product-bound crystal structure of the enzyme, revealing two distinct carbohydrate-binding sites in a trimeric β-helix architecture, which provide the first direct evidence for a second, non-catalytic, carbohydrate-binding site in bacteriophage-derived polysaccharide depolymerases. We found a bacteriophage-derived polysaccharide lyase that depolymerizes the K1 CPS into fragments of 1–3 repeating trisaccharide units with the retention of the pyruvylation and O-acetylation, and thus the important antigenic determinants of intact K1 CPS. pneumoniae-infected mice with the wild-type and mutant enzymes. We also performed site-directed mutagenesis, enzyme kinetic analysis, phage absorption and infectivity studies, and treatment of the K. The biochemical and biophysical properties of the enzyme were characterized and its crystal structures containing bound CPS products were determined. We analyzed the digested CPS products with NMR spectroscopy and mass spectrometry to reveal a bacteriophage-derived polysaccharide depolymerase specific to K1 CPS. To date, however, obtaining the fragments of K1 CPS that contain the pyruvylation and O-acetylation for generating glycoconjugate vaccines still remains a challenge. The pyruvylation and O-acetylation modifications on the K1 CPS are essential to the immune response induced by the CPS. This K1 CPS has been reported as a promising target for development of glycoconjugate vaccines against K. Patients with PLA often have serious complications, ultimately leading to a mortality of ~ 5%. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation.K1 capsular polysaccharide (CPS)-associated Klebsiella pneumoniae is the primary cause of pyogenic liver abscesses (PLA) in Asia. The University of California is an Equal Opportunity/Affirmative Action Employer. Strong communication skills both verbally and written needed to interact professionally and effectively. Theoretical knowledge of inorganic chemistry.ĭemonstrated use of computer software such as Excel, Word, Kaleidagraph, and Adobe Illustrator.Ībility to perform repetitive tasks meticulously Provide training and technical support to master s student and visiting scholars. Keep up-to-date safety records, and ensure that lab safety practices are observed. Responsible for lab management including ordering supplies, maintaining inventory and maintaining equipment. Complete data reduction and evaluate acceptability of acquired sample data. Prepare sample for strontium isotope analysis by dissolving carbonates. Determine the concentrations of various elements in pore waters using inductively coupled plasma optical emission spectroscopy (ICP-OES). Under general supervision perform sediment digestions to separate barite for sulfur and oxygen isotope analysis. Eligible ACCES or Special Selection clients should contact their Vocational Rehabilitation Counselor for Special Selection. All layoff applicants should contact their Employment Advisor. UCSD Layoff from Career Appointment or Special Selection: Apply by 4/15/16 for consideration with preference for rehire. PLEASE NOTE THAT THIS POSITION IS A 50% CAREER APPOINTMENT WITH BENEFITS.
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The ideal bond angles for this geometry are 109.5°.Īs an example of the tetrahedral structure,the figure at left shows the bond geometry of methane If the SN = 4, the observed structures are based on the tetrahedron.įor the case with all four electron domains bonding (then thereĪre four atoms bonded to the central atom), the positions of theīound atoms lie at the corners of a tetrahedron. Groups (as are all the examples mentioned above) this results in an ideal bond angle of 120°. For the case in which all electron groups are bonding The VSEPR model predicts a trigonal planar geometry, with electron groups oriented toward theĬorners of an equilateral triangle. The organic molecule formaldehyde (CH 2O), The VSEPR model predicts a linear geometry and aĮxamples of trigonal planar geometry (SN = 3) are provided by boron trichloride (BCl 3), That is, in each case they count as one electron group, and In the latter both count the same in determining the steric number SN (i.e. Note that the single bonds in the former and the double bonds We'll go on to look at SN = 5 and SN = 6 cases before turning to the use of molecular shapes to predict molecular polarity.Įxamples of linear geometry (SN = 2)are provided by beryllium dichloride (BeCl 2) andĬarbon dioxide (CO 2). Note that any atom obeying the octet rule will not have SN > 4.Īnd we'll begin by analyzing the possible molecular shapes for steric number equal to 2, 3 and 4. The value we assign to SN is the same as the number of electron domains (electron groups). (Tro's "electron groups") instead of electron pair in determining a value for SN. Or triple-bonded to the central atom, we sometimes use the term electron domain Since we end up counting a bonded atom only once, even if it is double. Tro's text refers to this count, what we are calling steric number, The number of lone pairs present on the central atom. This amounts simply to a count of the number of bonded atoms plus Number (abbreviated SN) for the central atom of a molecular structure. To do this, we apply the valence shell electron pair repulsion or VSEPR model. Shape of the molecule, or its three-dimensional structure. Molecular structure: The five basic shapesįor simple molecules, we can use them to predict the Single-central atom structures: The five basic shapes. GENERAL CHEMISTRY TOPICS Molecular structure But outside the game, you are all now free to play with the End Poem put it on posters, or t-shirts, or barn doors, set it to music, make it into an art film, whatever. This means that anyone can do whatever they want with the End Poem and its contents, because like Gough said, "It's a gift from the universe. So I give them the gift of the End Poem, and they give me the gift of continuing to distribute it around the world. So, what happened to Gough's ownership of The End poem in the end (No pun intended)? In the same post, Gough revealed that he has "liberated" The End Poem by giving it away to the public domain, after renouncing his rights to the copyright. And ever since it was written and added to the game, neither Mojang nor Microsoft have ever owned the rights to one of the main aspects of the highest-selling game of all time. The writer was then asked to sign the contract once again prior to Microsoft's acquisition of Mojang. Minecrafts Survival mode campaign infamously ends with the End Poem, authored by Julian Gough. This quote seems to be stating that since. However, when he was asked to sign a contract to give away the rights to The End poem to Mojang, he refused following failed negotiations with former CEO Carl Manneh. The End Poem begins with two unknown entities speaking about the player. 'minecraft end poem quote' poster by lrichardsonMinecraft end poem 1. Shop unique custom made Canvas Prints, Framed Prints, Posters, Tapestries, and more. Minecraft poem end poems other blogsMinecraft end poem Minecraft smp e19: the end poemPoem quote ending. Gough got the opportunity, and he soon wrote what later became the famous poem. High-quality Minecraft End Poem Wall Art designed and sold by artists. Julian Gough DecemAlso Read: Hunter x Hunter Manga Back on Hiatus as Yoshihiro Togashi is ‘Trying to Figure Out’ Publishing Schedule The controversial ending to Mojang Studios’ Minecraft has sparked plenty of conversation over the years. Here's the contract I never signed: /WA2FAizXkI In a recent blog post, Gough shared the origins of The End Poem and its journey over the course of the previous decade, starting with Minecraft creator Notch's tweet, asking for a writer to pen "a silly over-the-top out-of-nowhere text for when you win Minecraft.” The End Poem was written by Irish writer Julian Gough. The End Poem, created by Julian Gough, is. Since hair tests can identify substance use over the past 90 days, they give insight into how often an individual uses drugs.īecause hair drug testing offers a window into the past, employers typically use this testing method as part of their pre-employment screening process or as part of a random drug screening for employees.Īs Quest Diagnostics explains, a hair drug test requires about 100 – 120 strands of hair, or the equivalent of a one centimeter wide row of hairs. Hair drug tests are ideal for looking at long-term drug use. Opiates (codeine, morphine, 6-acetylmorphine)Įmployers may not always test for all of the drugs a hair drug test can detect.Once the hair has been collected, it’s sealed in foil and shipped for testing. The person collecting the hair will typically cut a 1.5 inch long sample. Hair drug testing (also called hair follicle drug testing) involves taking a sample of hair to look for patterns of drug use over the previous 90 days.ĭuring the test, around 100 strands of hair will be removed from the back of a person’s head. It only means that drugs were in their system within the few days leading up to the test. It’s important to note that a positive result on a urine drug test does not necessarily mean that the individual was on drugs at the time of the test. Urine drug testing is appropriate for pre-employment screening, random drug testing and post-accident drug testing.īecause urine drug tests detect substance use that occurred one to three days prior to the test being conducted, it may not be the best option for employers who need to have a better understanding of their candidate or employee’s longer-term drug use. Employers who use JDP’s drug screening services may request a custom panel to test only for specific substances. Once the person has collected their sample, the person administering the test will check to make sure it’s in the normal temperature range before sealing it for testing.Įmployers may not always test for every drug a urine test can detect. They’ll fill the cup with at least 45 milliliters (or a little over 3 tablespoons) of urine. The person taking the drug test will receive a specimen cup at the clinic conducting the test. Urine drug tests typically detect drug use that has occurred within the one to three days prior to the test being conducted. The most common form of drug testing, urine drug testing detects substances by analyzing a urine sample provided by a patient. These tests differ primarily in how they’re conducted and how far back they can detect drug use. Two of the most common drug testing methods are hair testing and urine testing. No matter the reason, employers have options for which drug testing method to use. Some test to maintain a safe work environment, some test to ensure substances won’t hinder productivity and some test to maintain compliance with various laws. By signing up you are agreeing to our Privacy Policy.Įmployers test job candidates and employees for drug use for a variety of reasons. By selecting the Alarms option and clicking on the New button, you can set alarms with custom names, reoccurrence, time, and actions, such as playing a sound/video or executing an application. Furthermore, the widget also enables you to set multiple alarms based on your needs. In addition to its built-in themes, the ClocX clock widget allows you to customize the appearance further by accessing the Options > Appearance tab. With a simple right-click on the clock, you can access several useful options, including the ability to keep it always on top, unmovable, or show a calendar. Download DS ClockĬlocX is a Windows 11/10 clock widget software that offers a customizable analog clock on top of your desktop. Don’t settle for a generic clock – download DS Clock and make it your own. To move the widget to a different position on your desktop, simply drag it where you want it. Additionally, you can tweak the sound settings and set the widget to launch upon Windows startup. If you want to keep your clock widget in a specific location on your desktop, you can even lock its position. DS Clock also offers font and color customization options for both the text and background, or you can keep the widget transparent. To customize the widget to your liking, simply right-click on the widget and select “Options.” From there, you can change the date and time format, choose your preferred separator, and even add a new time zone to the widget. Looking for a customizable clock widget for your Windows 11 or 10 desktop? Check out DS Clock, an easy-to-use option that displays the date and time by default upon download and installation. If you need “.Clocks,” you can download it from Microsoft Store. Clocks today and enjoy a streamlined and functional clock widget for your desktop. Plus, you can opt for a 24-hour clock display if that better suits your needs. Clocks app also includes customization options, allowing you to adjust the clock’s opacity and size, display seconds, and choose between analog and digital designs. Once the clock appears on your desktop, you can drag it to any location you prefer. To use a clock widget, simply download and install the app on your computer, then press the play button to activate it. This app offers five types of clock widgets with a clean and uncomplicated interface. Clocks, available for download from the Microsoft Store. Looking for a simple and free clock widget for your Windows desktop? Check out. You can get free Digital Clock 4 from here.Clocks Don’t settle for a boring default clock – download Digital Clock 4 and take control of your desktop’s appearance and functionality. Plus, check out the Experimental tab for even more options, such as full-screen mode and always-visible clock display. Under the Plugins tab, you can enhance the widget’s functionality with features like alarm settings, IP address display, and more. The widget is highly customizable, with options to adjust the appearance, including opacity and skin customization, as well as functional features like setting the time format and adjusting the space between digits. Right-clicking on the widget’s solid lines will bring up a menu of options, including the ability to change position, update, and exit. Simply install the widget on your PC, and it will appear in the top-left corner of your screen in digital form. Looking for a free and customizable clock widget for your Windows 11 or 10 desktop? Look no further than Digital Clock 4, available for download from. Desktop Clock Widgets for Windows 11 and 10 Digital Clock 4 In this gearupwindows article, we will cover a lot of free clock widgets that you can use on your Windows 10 or 11 desktop. Whether you want a simple digital clock or a widget with multiple features, we have got you covered. In this article, we will discuss some of the best free desktop clock widgets for Windows 11 and 10 that offer a range of features and styles to enhance your desktop experience. These widgets are convenient for users who need to keep track of time without constantly checking their phones or the taskbar clock. Luckily, there are plenty of clock widgets available for Windows 11 and 10 that provide a range of features and styles to suit everyone’s preferences.Ĭlock widgets are small applications that sit on top of your desktop, displaying the time and providing additional features such as alarms, timers, and world clocks. While the taskbar clock in Windows 11 or 10 displays the time, it may not be enough for users who require additional features or want to customize the clock’s appearance. If you’re someone who works on a computer all day, keeping track of time is crucial.
Pentagon: A five-sided polygon with five straight sides and five angles.Trapezoid: A four-sided polygon with one pair of parallel sides.Parallelogram: A four-sided polygon with two pairs of parallel sides.Rhombus: A four-sided polygon with equal sides but opposite angles are not 90 degrees.Rectangle: A four-sided polygon with two pairs of equal sides and angles of 90 degrees.Square: A four-sided polygon with equal sides and angles of 90 degrees.Triangle: A closed 2D shape with three straight sides and three angles.Here are some common geometric shapes arranged by the number of their sides (polygons): Shape Names by Number of Sides (Polygons) Shape Names by Number of Sides (Polygons).We’ll explore the various shapes as listed in the Table of Contents below. Overall, shape names are an important way to describe and classify different shapes, allowing us to better understand the world around us. Natural forms: Many natural forms, such as leaves or shells, can be described using shape names, such as ovate or conical.Artistic designs: Shape names are used in art and design to describe different shapes and patterns, such as a zigzag or a spiral.In this case, these names help us communicate and understand the properties of different objects, whether they are physical or abstract (nails, body shapes, tile, vases, rings, glasses, and more).
UPDATE: I got A$$ Creed 2 through their mobile app but I still can't log in, and thus still can't play it. I am able to log into Uplay on their website only because I'm not asked to use 2 factor authentication. Uplay is updated to the newest version (I downloaded a fresh installer to update) To get the verification code I use the Google Authenticator app on my phone. This means that in addition to your password, youll also. When I enter my admin username and password in Plesk I am presented with the Google Authenticator verification code prompt. As it turns out, Google Authenticator might not work if your system date and time settings are not set correctly. Google Authenticator adds an extra layer of security to your online accounts by adding a second step of verification when you sign in. (I've tried using both time zone settings windows has for my area) I have two step authentication extension enable in Plesk. The time on my PC and phone are both correct. Uplay offers no way for me to login without using one of those two methods and a quick google search shows multiple people have had this problem in the past but I haven't found solutions from those searches. Learn how to secure your Ubisoft account with 2-Step verification using a mobile app. I tried using my 1 time recovery codes but immediately ran into a problem, the verification box literally won't allow me to enter the last two numbers of the 1 time codes, so I can't use them. Encrypting your secrets is strongly recommended, especially if you are logged into a Google account. Always keep a backup of your secrets in a safe location. Use it to add an extra layer of security to your online accounts. a tartózkodási helyét), akkor elfordulhat, hogy nem kaphat ellenrz kódot SMS-ben. Authenticator generates two-factor authentication (2FA) codes in your browser. Ha a megszokottakhoz képest valami furcsát veszünk észre a bejelentkezés során (pl. You can also contact Ubisoft Support for more help. Lépjen Google-fiókja Kétlépcss azonosítás. Do you want to turn off 2-Step Verification on your Ubisoft account for any reason Follow the simple steps in this article to disable this security feature and access your account without any hassle. At first I thought I needed to update Uplay, so I did. Ha elvesztette biztonsági kódjait, visszavonhatja ket, és újakat kérhet. Entering my google authenticator codes always yields an "invalid code" error. Just grab your phone, install the Google Authenticator, synchronize your Uplay account with the app and you are good to go! For more detailed information about how it works, please head over to your Uplay account page on ubi.I wanted to log into Uplay to get Assassin's creed 2 since it's free right now. Enter the code shown in your authenticator app. Scan the QR code using your authenticator app. To see this option, youll need to have at least 2 recovery methods on your account. Setting up the two-factor authentication is pretty simple and doesn’t require any technical skills. Select Authenticator app for your 2-step verification method. Vous pouvez désactivez la Vérification en 2 étapes en cliquant sur ce lien en cliquant sur Gérer dans le client Uplay PC dans les Informations du Compte. This extra layer of security can be activated for free and prevents people from hijacking your account in case they have knowledge of your password, as they would need the additional security code which will then be only provided by the on your phone/tablet installed Google Authenticator app – available for iOS and Android. Ces codes vous seront demandés si Google Authenticator se désynchronise, si vous perdez votre téléphone ou si vous avez besoin de re-télécharger lapplication. Ubisoft has added a new security feature to protect your accounts even more, by using 2-Step Verification with Google Authenticator. Players who logged in into their Uplay accounts through the client today might have already seen this info message popping up. If you want even more features, you must purchase the commercial version of Waveform. It implements a brand-new audio engine, an improved interface, new audio effects, and several other improvements. Tracktion recently released Waveform 12.5 Free, the latest version of its free DAW. Its workflow is optimized for instrument plugins and soft synths, with intuitive MIDI sequencing. If you use many virtual instruments in your music, Waveform Free is probably the best free DAW for you. The tutorial videos cover all crucial topics, from basic setup and plugin installation to advanced audio editing and automation. It comes with an in-depth user manual that explains everything you need to know about music production on a computer.Īnd if you don’t care much about reading manuals, check out the training videos on Tracktion’s official website. Waveform Free was designed from the ground up to be intuitive for beginner producers who haven’t previously used a DAW. It can load virtual instruments and offers all the MIDI features required for sequencing VST plugins. If you’re producing electronic music, it’s easy to recommend Waveform Free as the perfect free digital audio workstation for the task. Tracktion recently updated Waveform Free to improve the video synchronization workflow further. This flexible freeware DAW can even load video files to synchronize your audio project to a movie scene or an animation. You can use Waveform Free to record, mix, and master your music projects from the ground up. Waveform Free lacks some of the features available in its premium counterpart, but all core functionality is included. The free DAW is available across all major operating systems, offering full VST plugin support and ReWire compatibility. It is the freeware version of Tracktion Corporation’s flagship digital audio workstation software, Waveform Pro. Waveform Free is a feature-packed free DAW. Pros: VST and ReWire support, fully functional free DAW, low upgrade price. Read how we rated the best digital audio workstations in the article below and decide which is best for you. That’s why I thoroughly tested every free DAW in this article and listed their pros and cons. We included mini-reviews of each digital audio workstation and an overview of its pros and cons.Įvery user has different demands, and it is impossible to say which free DAW is the best for everyone. It is an excellent choice for music producers who want to record multi-track audio without using virtual instruments or VST plugins.įind more info about the free DAWs below. If you don’t require VST plugin support, consider using Studio One Prime by PreSonus. Unfortunately, Cakewalk will soon be discontinued. The best free DAWs are Waveform Free and Cakewalk by BandLab. These are the 17 best free digital audio workstations you can download right now: The number of free digital audio workstations grows yearly, so I wrote this article to help you find the best free DAW in 2024.įree DAWs: The BEST Digital Audio Workstations (2024 UPDATE) And since I enjoy using freeware, I thoroughly tested every free DAW while doing my regular music production and sound design work. I used numerous digital audio workstations since I started making music in the early 2000s. There are only a few high-quality free digital audio workstations out there. However, your options are more limited if you’re building a music studio on a tight budget. You are probably familiar with Ableton Live, FL Studio, Studio One, Pro Tools, Cubase, Bitwig, and similar premium DAWs. There’s an abundance of commercial digital audio workstations on the market. It is also where you will use your favorite VST plugins. Your DAW software is where all the audio recording, audio editing, and mixing occur. A digital audio workstation is central to any music production software setup. Choosing a free DAW for your music studio is not easy. I think your explicit loop version has the same issue. * is much slower, as then the exp.() broadcast gets fused with the larger one, and exp is run N^2 times not 2N, per i. Notice that your first version constructs a vector exp.(1im*k_x.*x) and a transposed vector exp.(1im*k_y.*y) and then multiply them with * to make a new matrix, every time. I also tried other packages such as Strided, LoopVectorization, Tullio. However, the above Matlab function with elementwise operation can do the job in only 179.2 ms (more than 2 times faster than my Julia implementation). K_x = rand(ComplexF64, fsize) k_y = rand(ComplexF64, fsize) += f*exp(1im*k_y*y).*exp.(1im*k_x.*x)įunction combine_f_threads_(f, k_x, k_y, x, y)į_out = for id in CartesianIndices(f_out) Here is the code: function combine_f_mul(f, k_x, k_y, x, y)į_out = zeros(eltype(f),ndim1,ndim2,ndim3) What I don’t understand is that the explicit loop with the least allocation performs the worst. I wrote three versions: 1st with broadcast and matrix multiplication, 2nd with multithreads loop and broadcast, and 3rd with multithreads explicit loop. It basically takes a few input vectors and constructs a 3-dimensional matrix with summation along the first dimension of input arrays. Julia is mostly amazing except this function that I cannot get it to a similar speed as Matlab. This is equivalent to a bsxfun capability for matrix multiplication.I am recently porting some functions from Matlab to Julia. If a dimension is singleton then it is virtually expanded to the required size (i.e., equivalent to a repmat operation to get it to a conforming size but without the actual data copy). The first two dimensions must conform using the standard matrix multiply rules taking the transa and transb pre-operations into account, and dimensions 3:end must match exactly or be singleton (equal to 1). Which would be equivalent to the MATLAB m-code: i.e., MTIMESX treats these cases as arrays of 2D matrices and performs the operation on the associated parings. The remaining dimensions are duplicated and specify the number of individual matrix multiplies to perform for the result. Mtimesx('SPEEDOMP','OMP_SET_NUM_THREADS(4)') % sets SPEEDOMP mode with number of threads = 4įor nD cases, the first two dimensions specify the matrix multiply involved. 'N' or 'n' = No pre-operation (the default if trans_ is missing)ĭirective = One of the modes listed above, or other directivesĬ = mtimesx(A,B) % performs the calculation C = A * BĬ = mtimesx(A,'T',B) % performs the calculation C = A.' * BĬ = mtimesx(A,B,'g') % performs the calculation C = A * conj(B)Ĭ = mtimesx(A,'c',B,'C') % performs the calculation C = A' * B' Transb = A character indicating a pre-operation on B: Transa = A character indicating a pre-operation on A: Where transa, transb, and directive are the optional inputs: The general syntax is (arguments in brackets are optional): SPEEDOMP: Fastest BLAS, LOOPS, or LOOPOMP method even if it doesn't match MATLAB exactly SPEED: Fastest BLAS or LOOPS method even if it doesn't match MATLAB exactly MATLAB: Fastest BLAS or LOOPS method that matches MATLAB exactly (default) LOOPSOMP: Always uses OpenMP multi-threaded C loops if available LOOPS: Always uses C loops if available Can meet or beat MATLAB for speed in most cases Can match MATLAB results exactly or approximately as desired Utilizes BLAS calls, custom C loop code, or OpenMP multi-threaded C loop code Supports Transpose, Conjugate Transpose, and Conjugate pre-operations Supports multi-dimensional (nD, n>2) arrays directly MTIMESX is a fast general purpose matrix and scalar multiply routine that has the following features: |
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